In Vitro Potent Anticancer, Antifungal, and Antioxidant Efficacy of Walnut (Juglans regia L.) Genotypes

Walnuts are one of the healthiest foods in world because they best sources beneficial nutrients, minerals, and antioxidants. They also contain key energy. Despite several traditional uses, leaves Juglans regia L. have received little attention regarding phytochemical pharmacological potential. Thus, current study intended to find walnut genotypes with greatest antioxidant, anticancer, antifungal activity. The total polyphenolic, flavonoid, flavanol contents from 14 were determined. Genotypes that accumulate flavonoid/flavonol (99.8–111.93 mg/g quercetin equivalent (QE) 101.67–111.83 QE) showed significantly higher ferric reducing antioxidant potential (FRAP) activity (128.2–148.1 μM Fe2+/g dry weight (DW)] than other genotypes. Maximum divergence content (0.8–1.23 mg/g) was obtained by cluster analysis. active component, quercetin, measured using RP-HPLC. Moreover, extracts investigated for anticancer assays. We report significant leaf against Candida glabrata, albicans, tropicalis, 57.7–93.6%, 26.8–51.5%, 26.8–51.5% inhibition, respectively. most antiproliferative effect shown Opex Culchry, which exhibited 9.4% cell viability at a concentration 25 µL (0.75 mg) lung (A549) lines. Chenovo 2.9, 6.2, 2.2% viability, Culchry 2, 1.5, 2.4% Sulieman 7.6, 0.9, 7% colon (HCT116) results possess enormous as antioxidants, agents.